qPCR for target species detection and quantification
When the question involves a species or a predefined target, the analysis can take a targeted approach. Quantification depends on its applicability to the study scope.
A predefined target guides the analysis.
The starting point is to clarify what needs to be detected and what information the project requires.
qPCR is part of MetaGen’s environmental genomics solutions. Its suitability is discussed in light of the question, available material and analytical scope.
Targeted analysis or multiple taxa?
qPCR
An approach directed at a species or predefined target, for detection and quantification where applicable.
Metabarcoding
An approach for assessing multiple taxa from a sample, within the scope of the markers and references.
Explore metabarcoding ↗The approaches address different questions. The choice needs to align with the project objective.
Deliverables follow the scope.
Before analysis, the target of interest, sample type and expected information need to be aligned. The possibility of quantification must be assessed for the proposed scope.
Interpretation must consider the scope of the selected approach. Conclusions should not be extended to questions the analysis was not designed to answer.
A service area within a study.
A discussion with MetaGen helps connect the question with the possibilities of analysis. qPCR, environmental DNA, DNA barcoding, population genetics and genomics, and bioinformatics make up this set of solutions.
From sample to information ↗Which species or target guides your question?
Tell us about the study objective and context so we can discuss the suitability of the approach and the analytical scope.